USP21 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03947
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP21 (NP_036607.3). MPQASEHRLGRTREPPVN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit USP21 Antibody - Azide and BSA Free (NBP3-03947) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for USP21 Antibody - Azide and BSA Free
Western Blot: USP21 Antibody - Azide and BSA Free [USP21] -
Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] -
Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] -
Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for USP21 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: USP21
Long Name
Ubiquitin Specific Protease 21
Alternate Names
ESTM28, USP23, W53272
Gene Symbol
USP21
Additional USP21 Products
Product Documents for USP21 Antibody - Azide and BSA Free
Product Specific Notices for USP21 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov