USP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-88117
![Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117]](https://resources.bio-techne.com/images/products/USP1-Antibody-Immunohistochemistry-NB100-88117-img0003.jpg "Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human ubiquitin-specific-processing protease 1 using the numbering given in entry NP_003359.3 (GeneID 7398).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit USP1 Antibody - BSA Free (NB100-88117) is a polyclonal antibody validated for use in IHC and IP. Anti-USP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for USP1 Antibody - BSA Free
Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117]
Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: USP1 Antibody [NB100-88117]](https://resources.bio-techne.com/images/products/USP1-Antibody-Immunoprecipitation-NB100-88117-img0002.jpg "Immunoprecipitation: USP1 Antibody [NB100-88117]")
Immunoprecipitation: USP1 Antibody [NB100-88117]
Immunoprecipitation: USP1 Antibody [NB100-88117] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88117 used for IP at 3 mcg/mg lysate. USP1 was also immunoprecipitated by NB100-88116 and NB100-88118, which recognize other epitopes.![Immunoprecipitation: USP1 Antibody [NB100-88117]](https://resources.bio-techne.com/images/products/USP1-Antibody-Immunoprecipitation-NB100-88117-img0004.jpg "Immunoprecipitation: USP1 Antibody [NB100-88117]")
Immunoprecipitation: USP1 Antibody [NB100-88117]
Immunoprecipitation: USP1 Antibody [NB100-88117] - Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody NB100-88117 used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by rabbit anti-USP1 antibodies NB100-88116 and NB100-88118. For blotting immunoprecipitated USP1, NB100-88117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for USP1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000- 1:5000
Immunohistochemistry-Paraffin
1:1000- 1:5000
Immunoprecipitation
2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 or TrisEDTA pH 9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: USP1
Long Name
Ubiquitin Specific Protease 1
Alternate Names
Deubiquitinating Enzyme 1, Ubiquitin Carboxyl-Terminal Hydrolase 1, Ubiquitin Thioesterase 1, UBP
Entrez Gene IDs
7398 (Human)
Gene Symbol
USP1
UniProt
Additional USP1 Products
Product Documents for USP1 Antibody - BSA Free
Product Specific Notices for USP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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