U2AF35 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32515
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (98%), Chicken (98%), Rat (99%), Xenopus (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (97%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit U2AF35 Antibody - BSA Free (NBP1-32515) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-U2AF35 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for U2AF35 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-32515]](https://resources.bio-techne.com/images/products/U2AF35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32515-img0004.jpg "Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-32515]")
Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-32515]
Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515]](https://resources.bio-techne.com/images/products/U2AF35-Antibody-Immunohistochemistry-Paraffin-NBP1-32515-img0007.jpg "Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515]")
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515]
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.![Immunoprecipitation: U2AF35 Antibody [NBP1-32515]](https://resources.bio-techne.com/images/products/U2AF35-Antibody-Immunoprecipitation-NBP1-32515-img0013.jpg "Immunoprecipitation: U2AF35 Antibody [NBP1-32515]")
Immunoprecipitation: U2AF35 Antibody [NBP1-32515]
Immunoprecipitation: U2AF35 Antibody [NBP1-32515] - U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody. Western blot analysis was performed using U2AF35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.Applications for U2AF35 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Application Notes
WB: Detects an approx. 37 kDa protein.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: U2AF35
Alternate Names
FP793, human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712, RN, RNU2AF1,35-KD subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 small nuclear RNA auxillary factor 1, U2 snRNP auxiliary factor small subunit
Gene Symbol
U2AF1
UniProt
Additional U2AF35 Products
Product Documents for U2AF35 Antibody - BSA Free
Product Specific Notices for U2AF35 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.