U2AF2 Antibody (8E8R9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16432

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16432-100ul
NBP3-16432-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8E8R9 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all U2AF2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 376-475 of human U2AF2 (P26368). EVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit U2AF2 Antibody (8E8R9) (NBP3-16432) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for U2AF2 Antibody (8E8R9)

Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432]

Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432]

Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432] - Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432]

Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432]

Western Blot: U2AF2 Antibody (8E8R9) [NBP3-16432] - Western blot analysis of extracts of various cell lines, using U2AF2/U2AF2 Rabbit mAb (NBP3-16432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: U2AF2 Antibody (8E8R9) [NBP3-16432]

Immunoprecipitation: U2AF2 Antibody (8E8R9) [NBP3-16432]

Immunoprecipitation: U2AF2 Antibody (8E8R9) [NBP3-16432] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug U2AF2 antibody (NBP3-16432). Western blot was performed from the immunoprecipitate using U2AF2 antibody (NBP3-16432) at a dilition of 1:1000.
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Applications for U2AF2 Antibody (8E8R9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000-1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: U2AF2

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Alternate Names

hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit

Gene Symbol

U2AF2

Additional U2AF2 Products

Product Documents for U2AF2 Antibody (8E8R9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for U2AF2 Antibody (8E8R9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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