Tsukushi/TSK Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87959
![Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]](https://resources.bio-techne.com/images/products/Tsukushi-TSK-Antibody-Immunohistochemistry-Paraffin-NBP1-87959-img0010.jpg "Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Tsukushi/TSK Antibody - BSA Free (NBP1-87959) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Tsukushi/TSK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Tsukushi/TSK Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959]](https://resources.bio-techne.com/images/products/Tsukushi-TSK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87959-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959]")
Immunocytochemistry/ Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959]
Immunocytochemistry/Immunofluorescence: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human cell line A549 shows localization to nucleus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]](https://resources.bio-techne.com/images/products/Tsukushi-TSK-Antibody-Immunohistochemistry-Paraffin-NBP1-87959-img0012.jpg "Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]")
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.![Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]](https://resources.bio-techne.com/images/products/Tsukushi-TSK-Antibody-Immunohistochemistry-Paraffin-NBP1-87959-img0009.jpg "Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]")
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]
Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.![Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]](https://resources.bio-techne.com/images/products/Tsukushi-TSK-Antibody-Immunohistochemistry-Paraffin-NBP1-87959-img0011.jpg "Immunohistochemistry-Paraffin: Tsukushi/TSK Antibody [NBP1-87959]")
![Tsukushi/TSK Antibody - BSA Free Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]](https://resources.bio-techne.com/images/products/nbp1-87959_rabbit-polyclonal-tsukushi-tsk-antibody-6520251621386.jpg "Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]")
![Tsukushi/TSK Antibody - BSA Free Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]](https://resources.bio-techne.com/images/products/nbp1-87959_rabbit-polyclonal-tsukushi-tsk-antibody-6520251617114.jpg "Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]")
![Tsukushi/TSK Antibody - BSA Free Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]](https://resources.bio-techne.com/images/products/nbp1-87959_rabbit-polyclonal-tsukushi-tsk-antibody-65202516164216.jpg "Western Blot: Tsukushi/TSK Antibody - BSA Free [NBP1-87959]")
Applications for Tsukushi/TSK Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tsukushi/TSK
Tsukushi (TSK) is a secreted member of the SLRP (small leucine-rich proteoglycan) family of extracellular matrix molecules. It is named after a Japanese horsetail plant, which its expression pattern resembles in the chick primitive streak. Tsukushi binds BMP and Delta-1, contributes to formation of the primitive organizer and neural crest, and is upregulated in certain breast and bone cancer cell lines.
Long Name
Tsukushin Leucine-rich Repeat Containing Protein 54
Alternate Names
E2IG4, LRRC54, TSKU
Gene Symbol
TSKU
Additional Tsukushi/TSK Products
Product Documents for Tsukushi/TSK Antibody - BSA Free
Product Specific Notices for Tsukushi/TSK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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