TrkB [p Tyr707, p Tyr706] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54764
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Trk B (phospho Tyr706/Tyr707) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 706/tyrosine 707 (T-D-Y (p)-Y (p)-R-V)derived from Human Trk B.
Modification
p Tyr706, p Tyr707
Specificity
The antibody detects endogenous levels of TrkB only when phosphorylated at tyrosine 706 and tyrosine 707.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit TrkB [p Tyr707, p Tyr706] Antibody - BSA Free (NBP2-54764) is a polyclonal antibody validated for use in IHC and WB. Anti-TrkB Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TrkB [p Tyr707, p Tyr706] Antibody - BSA Free
Immunohistochemistry-Paraffin: TrkB [p Tyr706] Antibody [NBP2-54764] - Human brain tissue using Trk B [P-Tyr706/Tyr707) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western Blot: TrkB [p Tyr707, p Tyr706] Antibody - BSA Free [NBP2-54764] -
Effects of the ABA protocol on the protein levels of TrkB, the high affinity receptor for mBDNF, and on its downstream signaling effectors in the amygdala. Rats were exposed to the combination of food restriction and free access to the activity wheel and the analysis of phospho(p)TrkBY706 (A), TrkB receptor (B), pAktS473 (D), Akt (E), pERK2T185-Y187 (G), and ERK2 (H) were measured in the crude membrane fraction in the acute phase of the disease (PND42), and after a 7-day period of body weight recovery (PND49). Pearson’s product–moment correlation (r) analyses and linear regression analyses (R2) between body weight and pTrkBY706 (C), pAktS473 (F), and pERK2T185-Y187 (I) phosphorylation levels of CTRL and ABA rats at both time points are represented. In panel (L), representative immunoblots are shown for pTrkB Y706 (145 kDa), TrkB (145 kDa), pAktS473, Akt, pERK2T185-Y187, ERK2, and beta-Actin (43 kDa). Data are expressed as percentages of CTRL animals. Bar graphs represent the mean +/- SEM from five independent determinations for each experimental group. Unpaired Student’s T test. *p < 0.05, **p < 0.01, ***p < 0.05. CTRL or C, control; ABA or A, activity-based anorexia. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36570702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for TrkB [p Tyr707, p Tyr706] Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:200
Immunohistochemistry-Paraffin
1:10 - 1:500
Application Notes
This TrkB [p Tyr706] Antibody is validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 3 using NBP2-54764 in the following applications:
Formulation, Preparation, and Storage
Purification
Epitope affinity purified
Formulation
PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 °C.
Background: TrkB
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
Additional TrkB Products
Product Documents for TrkB [p Tyr707, p Tyr706] Antibody - BSA Free
Product Specific Notices for TrkB [p Tyr707, p Tyr706] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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