TRIM25 Antibody (4Q0D2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16382
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Q0D2 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM25 (Q14258). SPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TRIM25 Antibody (4Q0D2) (NBP3-16382) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TRIM25 Antibody (4Q0D2)
![Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382]](https://resources.bio-techne.com/images/products/TRIM25-Antibody-4Q0D2-Western-Blot-NBP3-16382-img0001.jpg "Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382]")
Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382]
Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of extracts of various cell lines, using TRIM25 Rabbit mAb (NBP3-16382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] -
Western Blot: TRIM25 Antibody (4Q0D2) [NBP3-16382] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] -
Western Blot: TRIM25 Antibody (4Q0D2) [TRIM25] - Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Applications for TRIM25 Antibody (4Q0D2)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TRIM25
Long Name
Tripartite Motif-containing Protein 25
Alternate Names
EFP, RNF147, ZNF147
Gene Symbol
TRIM25
Additional TRIM25 Products
Product Documents for TRIM25 Antibody (4Q0D2)
Product Specific Notices for TRIM25 Antibody (4Q0D2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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