Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # 685809

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Gly64-Asp245
Accession # AAC51762

Specificity

Detects human TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) TRANCE and approximately 25% cross-reactivity with recombinant human (rh) LIGHT is observed. No cross-reactivity with rh4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhOX40 LIgand, rhTNF-alpha, rhTRAIL, rhTWEAK, or rhVEGI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for TRANCE/TNFSF11/RANK L Antibody (685809) [DyLight 405]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB6263. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (685809) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (685809) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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