TOR/mTOR Antibody - BSA Free

Western Blot: TOR/mTOR Antibody [NB100-240]

Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: TOR/mTOR Antibody [NB100-240]

Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: TOR/mTOR Antibody - BSA Free [NB100-240] -

Promoting effect of total calystegine extract on hyperglycemia-mediated Akt/Pi3K/mTOR signaling pathway failure in HuASCs cells. (a) Histograms summarizing the relative gene expression of AKT, PI3K and MTOR mRNAs. (b) Relative Akt, Pi3K and mTOR protein expression levels normalized to GAPDH housekeeping protein and evaluated using western blot. (c) Representative immunoblots for each assayed protein detected by chemiluminescence. Representative data from three independent experiments are shown +/- SD (n = 3). An asterisk (*) indicates a comparison of the HG group to the untreated healthy cells. A hashtag (#) indicates a comparison of the HG group pre-treated with calystegines to the HG untreated healthy cells. */# p < 0.05, ** p < 0.01, ***/### p < 0.001. HuASCs_HE: Human ASCs healthy untreated cells; HuASCs_HG: hyperglycaemic human ASCs cells exposed to a high concentration of glucose. HuASCs_Caly 125 ug/mL: hyperglycaemic human ASCs cells pre-treated with 125 μg/mL calystegines extracted and exposed to a high concentration of glucose. HuASCs_Caly 250 ug/mL: hyperglycaemic human ASCs cells pre-treated with 250 μg/mL calystegines extracted and exposed to a high concentration of glucose. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35327652), licensed under a CC-BY license. Not internally tested by Novus Biologicals.