TLR9 Antibody (26C593.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24729
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Equine, Monkey, Primate
Cited:
Human, Mouse, Rat, Porcine, Canine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Dot Blot, Functional, In vitro assay, Knockdown Validated, CyTOF-ready
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Cytometric Bead Assay Standard, Flow Cytometry Control, In vitro assay, Functional Assay, IF/IHC, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 26C593.2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Reactivity Notes
Rhesus Monkey.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TLR9 Antibody (26C593.2) - BSA Free
Simple Western: TLR9 Antibody (26C593.2)BSA Free [NBP2-24729]
Simple Western: TLR9 Antibody (26C593.2) [NBP2-24729] - Lane view shows a specific band for TLR9 in 0.5 mg/ml of Ramos lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. Image using the Azide Free format of this antibody.Flow Cytometry: TLR9 Antibody (26C593.2) - BSA Free [NBP2-24729]
TLR9-Antibody-26C593-2-Flow-Cytometry-NBP2-24729-img0025.jpgApplications for TLR9 Antibody (26C593.2) - BSA Free
Application
Recommended Usage
Block/Neutralize
reported in scientific literature (PMID 25338738)
Dot Blot
reported in scientific literature (PMID 27248820)
ELISA
1:100 - 1:2000. Use reported in multiple pieces of scientific literature
Flow (Intracellular)
1:10 - 1:1000. Use reported in scientific literature (PMID 24986635)
Functional
reported in scientific literature (PMID 25411258)
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
reported in scientific literature (PMID 27744078)
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:10 - 1:500. Use reported in scientific literature (PMID 25871979)
In vitro assay
reported in scientific literature (PMID 25411258)
Knockdown Validated
reported in scientific literature (PMID 31655343)
Simple Western
30 ug/ml
Western Blot
2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Brain, Ramos lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, 30 ug/mL, apparent MW was 90 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Brain, Ramos lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, 30 ug/mL, apparent MW was 90 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 4 reviews rated 3.8 using NBP2-24729 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TLR9
Additional TLR9 Products
Product Documents for TLR9 Antibody (26C593.2) - BSA Free
Product Specific Notices for TLR9 Antibody (26C593.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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