Tie-2 Antibody (tek9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18614
![Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]](https://resources.bio-techne.com/images/products/Tie-2-Antibody-tek9-Flow-Cytometry-NBP1-18614-img0014.jpg "Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA, Sandwich ELISA, Flow Cytometry, CyTOF-ready
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # tek9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human soluble extracellular TIE-2
Specificity
The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Tie-2 Antibody (tek9) - Azide and BSA Free
Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]
Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).
Sandwich ELISA: Tie-2 Antibody (tek9) [NBP1-18614] - Quantification of soluble and cellular Tie-2 by sandwich ELISA. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTie-2 or Tie-2. For capturing anti-human Tie-2 Cl.16 NBP1-18616 was used, for the detection of a mixture of biotinylated anti-human Tie-2 Cl.2 NBP1-18612 and Cl.9 NBP1-18614.
Applications for Tie-2 Antibody (tek9) - Azide and BSA Free
Application
Recommended Usage
ELISA
1 - 2 ug/ml
Flow Cytometry
2 - 5 ug/ml
Western Blot
2 - 5 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tie-2
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Gene Symbol
TEK
Additional Tie-2 Products
Product Documents for Tie-2 Antibody (tek9) - Azide and BSA Free
Product Specific Notices for Tie-2 Antibody (tek9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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