Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: Thioredoxin-1 AntibodyBSA Free [NBP2-52983]

Western Blot: Thioredoxin Antibody [NBP2-52983] - Total protein from human HeLa cells, mouse 3T3 cells and rat PC12 cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um pore size PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Thioredoxin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-52983] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse intestine using rabbit polyclonal antibody against Thioredoxin (1:300 dilution). The signal was developed with HRP-secondary -DAB method and the sections were counter-stained using hematoxylin. The staining was found primarily in the epithelial cells of the intestinal lumen with weak to negligible signal in the basal or muscular layers. Isolated epithelial cells depicted nuclear positivity also.

Flow Cytometry: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - An intracellular stain was performed on HeLa cells with Thioredoxin-1 Antibody NBP2-52983AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - Thioredoxin Antibody [NBP2-52983] - An intracellular stain was performed on U-87 MG cells with Thioredoxin Antibody NBP2-52983 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Thioredoxin-1 in A431 Human Cell Line.

Thioredoxin-1 was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-Thioredoxin-1 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-52983AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

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Applications for Thioredoxin-1 Antibody - BSA Free

Application

Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

1:250 - 1:500

Immunohistochemistry-Paraffin

1:250 - 1:500

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM]

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Gene Symbol

TXN

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Thioredoxin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Thioredoxin-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52983

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Thioredoxin-1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Thioredoxin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Western Blot: Thioredoxin-1 AntibodyBSA Free [NBP2-52983]

Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Flow Cytometry: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Flow Cytometry: Thioredoxin-1 Antibody - BSA Free [NBP2-52983]

Thioredoxin-1 in A431 Human Cell Line.

Applications for Thioredoxin-1 Antibody - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Thioredoxin-1

Alternate Names

Gene Symbol

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Thioredoxin-1 Antibody - BSA Free