TGF-beta 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AB-245-NA
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant amphibian TGF-beta 5
Gly271-Ser382
Accession # P16176
Gly271-Ser382
Accession # P16176
Specificity
Detects TGF‑ beta5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with the other TGF-beta variants is observed. Neutralizes the biological activity of recombinant amphibian TGF-beta 5, but not TGF-beta 1, porcine TGF-beta 1.2, TGF-beta 2, or TGF-beta 3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for TGF-beta 5 Antibody
TGF‑ beta5 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta5 Antibody.
Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Amphibian TGF-beta 5 (0.1 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-TGF-beta 5 Polyclonal Antibody (Catalog # AB-245-NA). The ND50 is typically 0.03-0.08 µg/mL.Applications for TGF-beta 5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
Sample: Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
Neutralization
Measured by its ability to neutralize TGF-beta 5 inhibition of IL-4-dependent proliferation in the HT-2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.03-0.08 µg/mL in the presence of 0.1 ng/mL Recombinant Amphibian TGF-beta 5 and 7.5 ng/mL Recombinant Mouse IL-4.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 5
TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.
Long Name
Transforming Growth Factor beta 5
Alternate Names
TGFbeta 5
Entrez Gene IDs
397778 (Amphibian)
Gene Symbol
TGFB1.L
UniProt
Additional TGF-beta 5 Products
Product Documents for TGF-beta 5 Antibody
Product Specific Notices for TGF-beta 5 Antibody
For research use only
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