Detects TGF‑ beta2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 2% cross‑reactivity with TGF-beta 1, TGF-beta 3, and TGF-beta 5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF‑ beta2 Antibody

TGF-beta 2 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 2/Antibody.

Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-TGF-beta 2/Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-302-NA). The ND₅₀ is typically 0.0100-0.100 µg/mL.

Human TGF-beta 2 ELISA Standard Curve

Recombinant Human TGF-beta 2 (Catalog # 302-B2) was serially diluted and captured by Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF-beta 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-302-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for TGF‑ beta2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TGF-beta 2 (Catalog # 302-B2)

Neutralization

Measured by its ability to neutralize TGF‑ beta2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.0100-0.100 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta2 and 7.5 ng/mL Recombinant Mouse IL‑4.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 2

TGF- beta 2 (transforming growth factor beta 2) is a highly pleiotropic cytokine with important functions during cardiac, lung, craniofacial, limb, eye, ear, and urogenital system development. It forms a latent complex with LAP and one of the LTBP TGF-beta binding proteins. The active protein is released by Plasmin, MMP, Thrombospondin 1, or Integrin action. TGF-beta 2 signals through TGF-beta RII, Betaglycan, and either TGF-beta RI/ALK-5 or ALK-1.

Long Name

Transforming Growth Factor beta 2

Alternate Names

TGFB2, TGFbeta 2

Entrez Gene IDs

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Gene Symbol

TGFB2

Additional TGF-beta 2 Products

Product Documents for TGF‑ beta2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for TGF‑ beta2 Antibody

For research use only

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