TGF-beta Antibody (1D11.16.8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07718
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Hamster, Monkey
Applications
Western Blot, ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Functional Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1D11.16.8
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Bovine TGF-beta protein
Localization
Cytoplasm and extracellular (secreted)
Specificity
This monoclonal antibody recognizes TGF beta 1, 2 and 3. Three TGFs inhibit the growth of epithelial cells and stimulate the growth of mesenchymal cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08710)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TGF-beta Antibody (1D11.16.8)
TGF-beta Antibody (1D11.16.8) - Azide and BSA Free
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TGF beta Mouse Monoclonal Antibody (1D11.16.8).
TGF-beta Antibody (1D11.16.8) - Azide and BSA Free
Formalin-fixed, paraffin-embedded human Salivary Gland Carcinoma stained with TGF beta Mouse Monoclonal Antibody (1D11.16.8).Applications for TGF-beta Antibody (1D11.16.8)
Application
Recommended Usage
ELISA
0.1-0.4 ug/ml
Immunocytochemistry/ Immunofluorescence
8-25 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Cytokine Neutralization: Order Ab without BSA/Azide.
ELISA: 0.1-0.4ug/ml - Detection. ELISA: 0.1-0.4ug/ml - Detection, 2-8ug/ml - Capture.
Functional Assay: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
ELISA: 0.1-0.4ug/ml - Detection. ELISA: 0.1-0.4ug/ml - Detection, 2-8ug/ml - Capture.
Functional Assay: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TGF-beta
TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.
Long Name
Transforming Growth Factor beta
Alternate Names
TGFbeta
Gene Symbol
TGFB1
UniProt
Additional TGF-beta Products
Product Documents for TGF-beta Antibody (1D11.16.8)
Product Specific Notices for TGF-beta Antibody (1D11.16.8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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