TFIIE beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20610
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TFIIE beta. The exact sequence is proprietary.
Reactivity Notes
Bovine (87%), Mouse (86%), Rat (86%), Pig (87%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit TFIIE beta Antibody (NBP2-20610) is a polyclonal antibody validated for use in WB, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TFIIE beta Antibody
![Western Blot: TFIIE beta Antibody [NBP2-20610]](https://resources.bio-techne.com/images/products/TFIIE-beta-Antibody-Western-Blot-NBP2-20610-img0001.jpg "Western Blot: TFIIE beta Antibody [NBP2-20610]")
Western Blot: TFIIE beta Antibody [NBP2-20610]
Western Blot: TFIIE beta Antibody [NBP2-20610] - Sample (30 ug of whole cell lysate) A: Jurkat nucleus 10% SDS PAGE gel, diluted at 1:5000.
Chromatin Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] -
TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 ug of TFIIE beta antibody (NBP2-20610) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (NBP2-20610) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Applications for TFIIE beta Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TFIIE beta
Alternate Names
FE, General transcription factor IIE subunit 2, general transcription factor IIE, polypeptide 2 (beta subunit, 34kD), general transcription factor IIE, polypeptide 2, beta 34kDa, TF2E2TFIIE-beta, TFIIE-B, transcription initiation factor IIE subunit beta
Gene Symbol
GTF2E2
UniProt
Additional TFIIE beta Products
Product Documents for TFIIE beta Antibody
Product Specific Notices for TFIIE beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.