TFEB Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12758

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TFEB Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 325 and 375 of human Transcription Factor EB using the numbering given in entry NP_009093.1 (GeneID 7942).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TFEB Antibody - BSA Free (NBP2-12758) is a polyclonal antibody validated for use in WB and IP. Anti-TFEB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TFEB Antibody - BSA Free

Western Blot: TFEB Antibody [NBP2-12758]

Western Blot: TFEB Antibody [NBP2-12758]

Western Blot: TFEB Antibody [NBP2-12758] - Detection of Human TFEB by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.
Immunoprecipitation: TFEB Antibody [NBP2-12758]

Immunoprecipitation: TFEB Antibody [NBP2-12758]

Immunoprecipitation: TFEB Antibody [NBP2-12758] - Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody NBP2-12758 used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by a different rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody (NBP2-77360). For blotting immunoprecipitated TFEB, NBP2-77360 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for TFEB Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Gene Symbol

TFEB

UniProt

NP_009093.1 (Human)

Additional TFEB Products

Product Documents for TFEB Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFEB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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