TCR beta Antibody (H57-597) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20118CL1
Recombinant Monoclonal Antibody
![TCR beta Antibody (H57-597) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp3-20118cl1_rabbit-tcr-beta-mab-h57-597-corafluor-1-299202514593416.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # H57-597
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for TCR beta Antibody (H57-597) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for TCR beta Antibody (H57-597) [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-20118. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (H57-597) [CoraFluor™ 1]
Product Specific Notices for TCR beta Antibody (H57-597) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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