TCR beta Antibody (8A3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22486
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate, Yeast
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8A3
Concentration
0.15 mg/ml
Product Specifications
Immunogen
Human TCR beta chain constant region
Reactivity Notes
Primate reactivity reported in scientific literature (PMID: 7499875). Mouse reactivity reported in scientific literature (PMID: 21423155). Yeast reactivity reported in scientific literature (PMID: 21159619). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TCR beta Antibody (8A3)
![Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486]](https://resources.bio-techne.com/images/products/TCR-beta-Antibody-8A3-Flow-Cytometry-NBP2-22486-img0001.jpg "Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486]")
Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486]
Flow Cytometry: TCR beta Antibody (8A3) [NBP2-22486] - Analysis of TCR Beta F1 in Jurkat cells (green) compared to an isotype control (blue).
Immunohistochemistry: TCR beta Antibody (8A3) [NBP2-22486] -
High HLA-DQA1 expression in RCC promotes antigen presentation and cytotoxic activity. The IHC images were taken at ×200 magnification. (A) Differential analysis of TIICs showed that CD4+ T, CD8+ T and macrophages M1 were more expressed in RCC, while macrophages M0 was more expressed in LCC. (B) CD83, a marker of mature DCs, was used for IHC staining and it was highly expressed in tumor tissues of RCC but not LCC. (C) TCR alpha beta and TCR gamma delta were both more expressed in RCC than in LCC. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35936748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for TCR beta Antibody (8A3)
Application
Recommended Usage
Flow Cytometry
2 ug
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 0.5% BSA
Preservative
0.1% Sodium Azide
Concentration
0.15 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TCR beta
Alternate Names
T cell receptor beta chain, TCRB, TCRbeta, Tib
Gene Symbol
Tcrb
Additional TCR beta Products
Product Documents for TCR beta Antibody (8A3)
Product Specific Notices for TCR beta Antibody (8A3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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