TCF7/TCF1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57570
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TCF7/TCF1 Antibody - BSA Free (NBP2-57570) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TCF7/TCF1 Antibody - BSA Free
![Western Blot: TCF7/TCF1 Antibody [NBP2-57570]](https://resources.bio-techne.com/images/products/TCF7-TCF1-Antibody-Western-Blot-NBP2-57570-img0002.jpg "Western Blot: TCF7/TCF1 Antibody [NBP2-57570]")
Western Blot: TCF7/TCF1 Antibody [NBP2-57570]
Western Blot: TCF7/TCF1 Antibody [NBP2-57570] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.![Immunocytochemistry/ Immunofluorescence: TCF7/TCF1 Antibody [NBP2-57570]](https://resources.bio-techne.com/images/products/TCF7-TCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57570-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TCF7/TCF1 Antibody [NBP2-57570]")
Immunocytochemistry/ Immunofluorescence: TCF7/TCF1 Antibody [NBP2-57570]
Immunocytochemistry/Immunofluorescence: TCF7/TCF1 Antibody [NBP2-57570] - Staining of human cell line Hep G2 shows localization to nucleoplasm.![TCF7/TCF1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TCF7/TCF1 Antibody - BSA Free [NBP2-57570]](https://resources.bio-techne.com/images/products/nbp2-57570_rabbit-polyclonal-tcf7-tcf1-antibody-2562025913961.jpg "Chromatin Immunoprecipitation-exo-Seq: TCF7/TCF1 Antibody - BSA Free [NBP2-57570]")
Chromatin Immunoprecipitation-exo-Seq: TCF7/TCF1 Antibody - BSA Free [NBP2-57570]
ChIP-Exo-Seq composite graph for Anti-TCF7 (NBP2-57570) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for TCF7/TCF1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCF7/TCF1
Long Name
Transcription Factor 7
Alternate Names
TCF1
Gene Symbol
TCF7
Additional TCF7/TCF1 Products
Product Documents for TCF7/TCF1 Antibody - BSA Free
Product Specific Notices for TCF7/TCF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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