TBK1 Antibody (24GB4645)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51670

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NBP3-51670-100ul
NBP3-51670-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4645

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TBK1 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human TBK1 (Uniprot: Q9UHD2)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBK1 Antibody (24GB4645)

TBK1 Antibody (24GB4645)

Flow Cytometry: TBK1 Antibody (24GB4645)[NBP3-51670] -

Validation of TBK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TBK1 antibody (1:2,000) and analyzed using BD flow cytometer.
TBK1 Antibody (24GB4645)

Flow Cytometry: TBK1 Antibody (24GB4645)[NBP3-51670] -

Flow cytometric analysis of TBK1expression in HepG2 cells using TBK1 antibody (1:2,000). Green, isotype control; red, TANK binding kinase 1.
TBK1 Antibody (24GB4645)

Western Blot: TBK1 Antibody (24GB4645)[NBP3-51670] -

Western blotting analysis using TBK1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for TBK1 Antibody (24GB4645)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TBK1

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase

Gene Symbol

TBK1

Additional TBK1 Products

Product Documents for TBK1 Antibody (24GB4645)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TBK1 Antibody (24GB4645)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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