TBC1D15 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36552
![Western Blot: TBC1D15 Antibody [NBP2-36552]](https://resources.bio-techne.com/images/products/TBC1D15-Antibody-Western-Blot-NBP2-36552-img0001.jpg "Western Blot: TBC1D15 Antibody [NBP2-36552]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 1 to 50 of human TBC1 Domain Family, Member 15 using the numbering given in entry NP_073608.4.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TBC1D15 Antibody - BSA Free (NBP2-36552) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TBC1D15 Antibody - BSA Free
Western Blot: TBC1D15 Antibody [NBP2-36552]
Western Blot: TBC1D15 Antibody [NBP2-36552] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: TBC1D15 Antibody [NBP2-36552]](https://resources.bio-techne.com/images/products/TBC1D15-Antibody-Immunoprecipitation-NBP2-36552-img0002.jpg "Immunoprecipitation: TBC1D15 Antibody [NBP2-36552]")
Immunoprecipitation: TBC1D15 Antibody [NBP2-36552]
Immunoprecipitation: TBC1D15 Antibody [NBP2-36552] - Detection of human TBC1D15 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D15 antibody NBP2-36552 used for IP at 6 ug per reaction. TBC1D15 was also immunoprecipitated by rabbit anti-TBC1D15 antibody NBP2-36553. For blotting immunoprecipitated TBC1D15, NBP2-36552 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for TBC1D15 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TBC1D15
Alternate Names
domain family member 15, TBC1 domain family, member 15
Gene Symbol
TBC1D15
UniProt
Additional TBC1D15 Products
Product Documents for TBC1D15 Antibody - BSA Free
Product Specific Notices for TBC1D15 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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