Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20162
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # TOC1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing a mouse with electro eluted cross-linked Tau dimers.
Epitope
This antibody is specific for amino acids 209-224 of the Tau Oligomeric complex.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Novus Biologicals Rabbit Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free (NBP3-20162) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images
Western Blot: Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free [NBP3-20162] -
Western Blot: Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free [NBP3-20162] - Kelly(A) (0.5ug/ml) and U251(B) (0.5ug/ml) cells lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of TOC1 (NBP3-20162) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free [NBP3-20162] -
Immunocytochemistry/Immunofluorescence: Tau Oligomeric Complex Antibody (TOC1) - Chimeric - Azide and BSA Free [NBP3-20162] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of TOC1 (NBP3-20162) (1:1000 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20162, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Tau Oligomeric Complex
Alternate Names
Tau Oligomeric Complex 1, TOC1, TOC-1
Additional Tau Oligomeric Complex Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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