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Tau Antibody (HL2736) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45160

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-45160

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2736

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human Tau.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Tau Antibody (HL2736) - Azide and BSA Free (NBP3-45160) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Tau Antibody (HL2736) - Azide and BSA Free

Tau Antibody (HL2736) - Azide and BSA Free

Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] -

Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Tau Antibody (HL2736) - Azide and BSA Free

Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] -

Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.
Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.
Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.
Tau Antibody (HL2736) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] -

Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Tau Antibody (HL2736) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

Additional Tau Products

Product Documents for Tau Antibody (HL2736) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tau Antibody (HL2736) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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