Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45157
Recombinant Monoclonal Antibody
![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg "ELISA: Rabbit Tau [p Thr181] mAb (HL2497) -")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2497
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr181 of human Tau. The exact sequence is proprietary.
Modification
p Thr181
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free (NBP3-45157) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free
![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -")
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg "Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -")
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg "Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -")
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -
Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg "ELISA: Rabbit Tau [p Thr181] mAb (HL2497) -")
![Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg "Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] -")
Applications for Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tau
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
Additional Tau Products
Product Documents for Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free
Product Specific Notices for Tau [p Thr181] Antibody (HL2497) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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