Syntaxin 7 Antibody (CL0257) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43939

Novus Biologicals, part of Bio-Techne
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NBP3-43939
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BSA Free
NBP2-52893
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0257

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Syntaxin 7 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O15400, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Syntaxin 7 Antibody (CL0257) - Azide and BSA Free (NBP2-52893) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Syntaxin 7 Antibody (CL0257) - Azide and BSA Free

Syntaxin 7 Antibody (CL0257) - Azide and BSA Free Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP3-43939]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP3-43939]

Analysis in human lymph node and testis tissues using NBP3-43939 antibody. Corresponding Syntaxin 7 RNA-seq data are presented for the same tissues.
Syntaxin 7 Antibody (CL0257) - Azide and BSA Free Western Blot: Syntaxin 7 Antibody [NBP3-43939]

Western Blot: Syntaxin 7 Antibody [NBP3-43939]

Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
Syntaxin 7 Antibody (CL0257) - Azide and BSA Free Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP3-43939]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP3-43939]

Staining of human kidney shows strong apical membrane positivity in cells in proximal tubules.
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Applications for Syntaxin 7 Antibody (CL0257) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin 7

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Alternate Names

STX7

Gene Symbol

STX7

Additional Syntaxin 7 Products

Product Documents for Syntaxin 7 Antibody (CL0257) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Syntaxin 7 Antibody (CL0257) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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