SUPT5H Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83508
![Western Blot: SUPT5H Antibody [NBP1-83508]](https://resources.bio-techne.com/images/products/SUPT5H-Antibody-Western-Blot-NBP1-83508-img0006.jpg "Western Blot: SUPT5H Antibody [NBP1-83508]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFL
Reactivity Notes
Reactivity reported in scientific literature (PMID: 22864569)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SUPT5H Antibody - BSA Free (NBP1-83508) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SUPT5H Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SUPT5H Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody [NBP1-83508]](https://resources.bio-techne.com/images/products/SUPT5H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83508-img0010.jpg "Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody [NBP1-83508]")
Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody [NBP1-83508]
Immunocytochemistry/Immunofluorescence: SUPT5H Antibody [NBP1-83508] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.![SUPT5H Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SUPT5H Antibody - BSA Free [NBP1-83508]](https://resources.bio-techne.com/images/products/nbp1-83508_rabbit-polyclonal-supt5h-antibody-2562025913957.jpg "Chromatin Immunoprecipitation-exo-Seq: SUPT5H Antibody - BSA Free [NBP1-83508]")
Chromatin Immunoprecipitation-exo-Seq: SUPT5H Antibody - BSA Free [NBP1-83508]
ChIP-Exo-Seq composite graph for Anti-SUPT5H (NBP1-83508) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.![Western Blot: SUPT5H Antibody [NBP1-83508]](https://resources.bio-techne.com/images/products/SUPT5H-Antibody-Western-Blot-NBP1-83508-img0008.jpg "Western Blot: SUPT5H Antibody [NBP1-83508]")
Western Blot: SUPT5H Antibody [NBP1-83508]
Western Blot: SUPT5H Antibody [NBP1-83508] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![SUPT5H Antibody - BSA Free Immunohistochemistry-Paraffin: SUPT5H Antibody - BSA Free [NBP1-83508]](https://resources.bio-techne.com/images/products/nbp1-83508_rabbit-polyclonal-supt5h-antibody-842025177541.jpg "Immunohistochemistry-Paraffin: SUPT5H Antibody - BSA Free [NBP1-83508]")
![Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]](https://resources.bio-techne.com/images/products/SUPT5H-Antibody-Immunohistochemistry-Paraffin-NBP1-83508-img0011.jpg "Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]")
![Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]](https://resources.bio-techne.com/images/products/SUPT5H-Antibody-Immunohistochemistry-Paraffin-NBP1-83508-img0012.jpg "Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]")
Applications for SUPT5H Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SUPT5H
Alternate Names
DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, FLJ34157, SPT5hSPT5, SPT5HTat-cotransactivator 1 protein, suppressor of Ty (S.cerevisiae) 5 homolog, suppressor of Ty 5 homolog (S. cerevisiae), Tat-CT1, Tat-CT1 protein, transcription elongation factor SPT5
Entrez Gene IDs
6829 (Human)
Gene Symbol
SUPT5H
UniProt
Additional SUPT5H Products
Product Documents for SUPT5H Antibody - BSA Free
Product Specific Notices for SUPT5H Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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