Strep II Tag Antibody (517) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43735
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 517
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunogen Amino acid sequence - ASWSHPQFEKGA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Strep II Tag Antibody (517) - Azide and BSA Free (NBP2-43735) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-Strep II Tag Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Strep II Tag Antibody (517) - Azide and BSA Free
![Western Blot: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0003.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735]")
Western Blot: Strep II Tag Antibody (517) [NBP2-43735]
Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.![Western Blot: Strep II Tag Antibody (517) [NBP2-43735]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0004.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735]")
Western Blot: Strep II Tag Antibody (517) [NBP2-43735]
Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0004.jpg
Western Blot: Strep II Tag Antibody (517) [NBP2-43735] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for Strep II Tag Antibody (517) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:1000-1:10000
Reviewed Applications
Read 2 reviews rated 5 using NBP2-43735 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Strep II Tag
Additional Strep II Tag Products
Product Documents for Strep II Tag Antibody (517) - Azide and BSA Free
Product Specific Notices for Strep II Tag Antibody (517) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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