STEAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48437
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an N-terminal portion of the human STEAP protein [UniProt Q9UHE8].
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit STEAP1 Antibody (NBP3-48437) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for STEAP1 Antibody
Immunohistochemical Staining of STEAP1 in Human Prostate
Analysis of a FFPE tissue section of human prostate using 1:200 dilution of STEAP1 antibody (NBP3-48437). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
STEAP1 in U-87 MG Human Cell Line.
STEAP1 was detected in immersion fixed U-87 MG human glioblastoma cell line using Rabbit anti-STEAP1 Antigen Affinity Purified Polyclonal Antibody (Catalog # NBP3-48437) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Detection of STEAP1 in U-87 MG Human Cell Line by Flow Cytometry.
An intracellular stain was performed on U-87 MG human brain glioblastoma cell line with Rabbit anti-STEAP1 Affinity-purified Polyclonal Antibody (Catalog # NBP3-48437, blue histogram) or matched control antibody (NBP2-24891, orange histogram) at 2 µg/mL for 30 minutes at RT.Applications for STEAP1 Antibody
Application
Recommended Usage
Flow (Intracellular)
1 - 2 ug/ml
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:200 - 1:300
Immunohistochemistry-Paraffin
1:200 - 1:300
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: STEAP1
Long Name
Six Transmembrane Epithelial Antigen of the Prostate 1
Alternate Names
PRSS24
Gene Symbol
STEAP1
Additional STEAP1 Products
Product Documents for STEAP1 Antibody
Product Specific Notices for STEAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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