SSEA-4 Antibody (MC-813-70) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1435PECY55
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Applications for SSEA-4 Antibody (MC-813-70) [PE/Cy5.5]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: SSEA-4
SSEAs were originally identified by monoclonal antibodies recognizing defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. They are often used as markers for stem cell differentiation. CD15/Lewis X/SSEA-1 is expressed on murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and primordial germ cells. SSEA-3 and SSEA-4 are synthesized during oogenesis and is present on oocyte, zygote, and early cleavage-stage embryo membranes. Mouse CD15/Lewis X/SSEA-1 expression decreases with differentiation as SSEA-3 and SSEA-4 expression increase. In contrast, human EC and ES cells express SSEA-3 and SSEA-4, and differentiation is accompanied by an upregulation of CD15/Lewis X/SSEA-1 and down-regulation of SSEA-3 and SSEA-4.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Additional SSEA-4 Products
Product Documents for SSEA-4 Antibody (MC-813-70) [PE/Cy5.5]
Product Specific Notices for SSEA-4 Antibody (MC-813-70) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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