SP1 Antibody (24GB7095)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51815

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NBP3-51815-100ul
NBP3-51815-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 24GB7095

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human SP1 (Uniprot: P08047)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SP1 Antibody (24GB7095)

SP1 Antibody (24GB7095)

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (24GB7095)[NBP3-51815] -

SP1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: SP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
SP1 Antibody (24GB7095)

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (24GB7095)[NBP3-51815] -

Immunocytochemical staining of HAP-1 cells with SP1 antibody (1:1,000) . Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
SP1 Antibody (24GB7095)

Flow Cytometry: SP1 Antibody (24GB7095)[NBP3-51815] -

Validation of SP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SP1 antibody (1:2,000) and analyzed using BD flow cytometer.
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Applications for SP1 Antibody (24GB7095)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody (24GB7095)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SP1 Antibody (24GB7095)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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