SOX6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20458
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (98%), Chicken (90%), Mouse (96%), Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length human SOX6 Recombinant protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SOX6 Antibody
Western Blot: SOX6 Antibody [NBP2-20458]
Western Blot: SOX6 Antibody [NBP2-20458] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.Immunoprecipitation: SOX6 Antibody [NBP2-20458]
Immunoprecipitation: SOX6 Antibody [NBP2-20458] - IP samples: HeLa whole cell extract A. Control with 5 ug of preimmune Rabbit IgG B. Immunoprecipitation of SOX6 protein by 5 ug SOX6 antibody [N1C3] 7.5 % SDS-PAGE. The immunoprecipitated SOX6 protein was detected by SOX6 antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Western Blot: SOX6 Antibody [NBP2-20458] -
Validation of targeted binding relationship between miR-499-5p and target genes. (A) The relative expression levels of SOX6, PPP3CB, and FNIP1 in miR-499-5p overexpressing CPMs and negative control by qRT-PCR; (B) Results of luciferase reporter assay for SOX6 and miR-499-5p; miR-499-5p binding site mutant and wildtype vector of SOX6’s 3'UTR were used. (C) Results of the luciferase reporter as say for PPP3CB/miR-499-5p and FNIP1/miR-499-5p; only wildtype vectors were used. All results are shown as mean +/- SD. **p < 0.01 and *p < 0.05 as compared with control. (D) The protein levels of SOX6 in miR-499-5p overexpressing CPMs and negative control by western bolting. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36186474), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SOX6 Antibody
Application
Recommended Usage
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SOX6
Additional SOX6 Products
Product Documents for SOX6 Antibody
Product Specific Notices for SOX6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.