As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.

This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For SOX2 Antibody NB110-37235, the exact immunogen is available to me in our internal notes. The UniProt Blast of this gave a 100% match with the (again, unfortunately unreviewed) horse entries F6PUQ2 and B6VA41. A 100% match indicates that the antibody should be suitable for you. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Can you advise me the pH value for the citrate buffer?

For the validation of this antibody in IHC-P, we performed antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0. Please note that Domyan et al 2011 from University of Wisconsin-Madison (USA) have cited the use of this antibody in IHC-P application in their experiments published in - Development (2011) 138, 971-981 Biologists site.

We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.

For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.

SOX2 Antibody - BSA Free

Name: SOX2 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB110-37235
Rating: 4 (4 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-37235

(4)

Citations (44)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NB110-37235AF350

View Product

Alexa Fluor 405

NB110-37235AF405

View Product

Alexa Fluor 488

NB110-37235AF488

View Product

Alexa Fluor 532

NB110-37235AF532

View Product

Alexa Fluor 594

NB110-37235AF594

View Product

Alexa Fluor 647

NB110-37235AF647

View Product

Alexa Fluor 700

NB110-37235AF700

View Product

Alexa Fluor 750

NB110-37235AF750

View Product

Alexa Fluor Plus 405

NB110-37235AFP405

View Product

Alexa Fluor Plus 488

NB110-37235AFP488

View Product

Alexa Fluor Plus 555

NB110-37235AFP555

View Product

Alexa Fluor Plus 594

NB110-37235AFP594

View Product

Alexa Fluor Plus 647

NB110-37235AFP647

View Product

Alexa Fluor Plus 680

NB110-37235AFP680

View Product

Allophycocyanin

NB110-37235APC

View Product

Biotin

NB110-37235B

View Product

CoraFluor 1

NB110-37235CL1

View Product

DyLight 350

NB110-37235UV

View Product

DyLight 405

NB110-37235V

View Product

DyLight 488

NB110-37235G

View Product

DyLight 550

NB110-37235R

View Product

DyLight 594

NB110-37235DL594

View Product

DyLight 650

NB110-37235C

View Product

DyLight 680

NB110-37235FR

View Product

DyLight 755

NB110-37235IR

View Product

FITC

NB110-37235F

View Product

HRP

NB110-37235H

View Product

Janelia Fluor 525

NB110-37235JF525

View Product

Janelia Fluor 549

NB110-37235JF549

View Product

Janelia Fluor 646

NB110-37235JF646

View Product

Janelia Fluor 669

NB110-37235JF669

View Product

mFluor Violet 450 SE

NB110-37235MFV450

View Product

mFluor Violet 500 SE

NB110-37235MFV500

View Product

mFluor Violet 610 SE

NB110-37235MFV610

View Product

NB110-37235PE

View Product

PerCP

NB110-37235PCP

View Product

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Sheep

Cited:

Human, Mouse, Rat, Canine

Predicted:

Chicken (92%). Backed by our 100% Guarantee.

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, COMET

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SOX2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31690408).

Localization

Nuclear

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX2 Antibody - BSA Free

Detection of SOX2 in Mouse Brain Cortex via seqIF™ staining on COMET™

SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Brain Cortex using Rabbit Anti-Mouse SOX2, Monoclonal Antibody (Catalog # NB110-37235) at 1:125 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder.

Immunohistochemistry-Paraffin: SOX2 Antibody - BSA Free [NB110-37235]

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

View 8 more images

Applications for SOX2 Antibody - BSA Free

Application

Recommended Usage

Flow (Intracellular)

1:100

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50 - 1:250

Immunohistochemistry

1:125 - 1:250

Immunohistochemistry-Paraffin

1:125 - 1:250

Multiplex Immunofluorescence

1:125

Western Blot

0.5 ug/mL

Application Notes

This SOX2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot analysis, where a band is observed at ~40 kDa representing the SOX2 protein.

Reviewed Applications

Read 4 reviews rated 4.3 using NB110-37235 in the following applications:

Immunocytochemistry (1 Review)
Western Blot (3 Reviews)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOX2

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Entrez Gene IDs

6657 (Human); 20674 (Mouse)

Gene Symbol

SOX2

UniProt

P48431

Additional SOX2 Products

Product Documents for SOX2 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for SOX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Related Products
Blogs

SOX2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-37235

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all SOX2 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Marker

Clonality

Host

Isotype

Scientific Data Images for SOX2 Antibody - BSA Free

Detection of SOX2 in Mouse Brain Cortex via seqIF™ staining on COMET™

Immunohistochemistry-Paraffin: SOX2 Antibody - BSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Applications for SOX2 Antibody - BSA Free

Flow (Intracellular)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Multiplex Immunofluorescence

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: SOX2

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional SOX2 Products

Product Documents for SOX2 Antibody - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for SOX2 Antibody - BSA Free