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SnoN Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87020

Novus Biologicals, part of Bio-Techne
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NBP1-87020
NBP1-87020-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEEMKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVSQSKELTKTEASKSISRQSEKAHSSGKLQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SnoN Antibody - BSA Free (NBP1-87020) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SnoN Antibody - BSA Free

SnoN Antibody - BSA Free Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-87020]

Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-87020]

Staining of human colon shows strong cytoplasmic positivity in glandular cells.
SnoN Antibody - BSA Free Western Blot: SnoN Antibody - BSA Free [NBP1-87020]

Western Blot: SnoN Antibody - BSA Free [NBP1-87020]

Analysis in human cell line NTERA-2.
SnoN Antibody - BSA Free Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-87020]

Immunohistochemistry-Paraffin: SnoN Antibody - BSA Free [NBP1-87020]

Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Applications for SnoN Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SnoN/Ski-like Protein

SnoN may have regulatory role in cell division or differentiation in response to extracellular signals

Long Name

SKI-like Oncogene

Alternate Names

Ski like Protein, SKIL, SNO, SnoA, SnoI

Gene Symbol

SKIL

Additional SnoN/Ski-like Protein Products

Product Documents for SnoN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SnoN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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