SMUG1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1414
Key Product Details
Species Reactivity
Human
Applications
Validated:
Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence PQAFLLGSIHEPA-C corresponding to N-Terminus according to NP_055126.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Goat SMUG1 Antibody (NB100-1414) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-SMUG1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SMUG1 Antibody
Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]
Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: SMUG1 Antibody [NB100-1414]
Flow Cytometry: SMUG1 Antibody [NB100-1414] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Immunocytochemistry/ Immunofluorescence: SMUG1 Antibody [NB100-1414]
Immunocytochemistry/Immunofluorescence: SMUG1 Antibody [NB100-1414] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for SMUG1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:128000
Application Notes
Use in Western blot has been reported in scientific literature (PMID: 19384898).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMUG1
Long Name
Single-strand Selective Monofunctional Uracil DNA Glycosylase 1
Alternate Names
EC 3.2.2, EC 3.2.2.-, FDG, HMUDG, MGC104370, single-strand selective monofunctional uracil DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase 1, UNG3
Entrez Gene IDs
23583 (Human)
Gene Symbol
SMUG1
UniProt
Additional SMUG1 Products
Product Documents for SMUG1 Antibody
Product Specific Notices for SMUG1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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