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SmarcAL1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20414

Novus Biologicals, part of Bio-Techne
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NBP2-20414

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SmarcAL1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SmarcAL1 Antibody - BSA Free (NBP2-20414) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SmarcAL1 Antibody - BSA Free

SmarcAL1 Antibody

Western Blot: SmarcAL1 Antibody [NBP2-20414] -

Western Blot: SmarcAL1 Antibody [NBP2-20414] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (NBP2-20414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SmarcAL1 Antibody - BSA Free

Western Blot: SmarcAL1 Antibody [NBP2-20414] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (NBP2-20414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SmarcAL1 Antibody - BSA Free

Immunoprecipitation: SmarcAL1 Antibody [NBP2-20414] -

SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments.
IP samples: A549 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of SMARCAL1 protein by 4 ug SmarcAL1 antibody (NBP2-20414)
5 % SDS-PAGE
The immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody (NBP2-20414) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for SmarcAL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SmarcAL1

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq]

Alternate Names

ATP-driven annealing helicase, EC 3.6.1, EC 3.6.4.-, HARPSWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A-like protein 1, HepA-related protein, HHARP, SIOD, SMARCA-like protein 1, subfamily a-like 1, Sucrose nonfermenting protein 2-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin

Gene Symbol

SMARCAL1

UniProt

Additional SmarcAL1 Products

Product Documents for SmarcAL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SmarcAL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.