Smad3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20411
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (99%), Canine (100%), Chicken (99%), Porcine (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Smad3. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (95%).
Localization
Cytoplasm (By similarity); Nucleus (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Smad3 Antibody - BSA Free (NBP2-20411) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Smad3 Antibody - BSA Free
Chromatin Immunoprecipitation: SMAD3 Antibody [NBP2-20411] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of SMAD3 antibody The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.
Western Blot: Smad3 Antibody [NBP2-20411] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (NBP2-20411) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Smad3 Antibody [NBP2-20411] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (NBP2-20411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Smad3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:1000-1:1000
Western Blot
1:1000-1:20000
Application Notes
ChIP, Flow- assay Assay-dependent dilution
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Smad3
Long Name
Mothers Against DPP Homolog 3
Alternate Names
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbol
SMAD3
UniProt
Additional Smad3 Products
Product Specific Notices for Smad3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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