SMAD1/5/8/9 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56656
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against two synthetic peptides corresponding to amino acids 19-33 (GWKQGDEEEKWAEKA) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1.
Specificity
The synthetic peptide immunogens used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMAD1/5/8/9 Antibody - BSA Free
![Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]](https://resources.bio-techne.com/images/products/SMAD1-5-8-9-Antibody-Western-Blot-NB100-56656-img0003.jpg "Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]")
Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]
Western Blot: SMAD1/5/8/9 Antibody [NB100-56656] - Analysis using SMAD1/5/8/9 antibody. Lysate from Saos-2 cells probed with SMAD antibody.
Western Blot: SMAD1/5/8/9 Antibody - BSA Free [NB100-56656] -
Radiation effects on TGF‐ beta receptor signaling via noncanonical pathway. (A) Smad1/5/8/9 and pSmad1/5/9 expression in cancer‐associated fibroblasts (CAF) cell lysates analyzed by western blot. (B) quantitative analyses of Smad1/5/8/9 expression in cell samples; (C) quantitative analyses of pSmad1/5/9 expression in cell samples. In B and C, data for pSmad1/5/9 represents mean (+/- SD) values from three different CAF donors. Statistical p‐values between CTR (nonirradiated) and irradiated CAFs were determined using ordinary one‐way ANOVA with Dunnett correction for multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38488488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SMAD1/5/8/9 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMAD1/5/8/9
Alternate Names
BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbol
SMAD1
UniProt
Additional SMAD1/5/8/9 Products
Product Documents for SMAD1/5/8/9 Antibody - BSA Free
Product Specific Notices for SMAD1/5/8/9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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