Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2. The immunogen is located within the first 50 amino acids of SKA2.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SKA2 Antibody - BSA Free

Western Blot: SKA2 AntibodyBSA Free [NBP1-76312]

Western Blot: SKA2 Antibody [NBP1-76312] - 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 ug/mL.

Applications for SKA2 Antibody - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.5 - 1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKA2

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

Alternate Names

FAM33A, family with sequence similarity 33, member A, FLJ12758, MGC110975, spindle and kinetochore associated complex subunit 2, spindle and kinetochore-associated protein 2, spindle and KT (kinetochore) associated 2

Gene Symbol

SKA2

UniProt

NP_872426 (Human)

Additional SKA2 Products

Product Documents for SKA2 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for SKA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

SKA2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76312

Key Product Details

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Format

Concentration

View all SKA2 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Scientific Data Images for SKA2 Antibody - BSA Free

Western Blot: SKA2 AntibodyBSA Free [NBP1-76312]

Applications for SKA2 Antibody - BSA Free

ELISA

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: SKA2

Alternate Names

Gene Symbol

UniProt

Additional SKA2 Products

Product Documents for SKA2 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for SKA2 Antibody - BSA Free