Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50949

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1540

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 1/SIRT1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-Sirtuin 1/SIRT1 (S47) (Uniprot: Q96EB6)

Modification

p Ser47

Specificity

This antibody detects endogenous levels of Sirtuin 1/SIRT1 only when phosphorylated at S47

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)[NBP3-50949] -

Immunocytochemical staining of HAP-1 cells with Sirtuin 1/SIRT1 antibody (1:1,000). Nuclei were stained blue with DAPI; Sirtuin 1/SIRT1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Flow Cytometry: Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)[NBP3-50949] -

Flow cytometric analysis of Sirtuin 1/SIRT1expression in HAP-1 cells using Sirtuin 1/SIRT1 antibody (1:2,000). Green, isotype control; red, Phospho-SIRT1 (S47).
Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Western Blot: Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)[NBP3-50949] -

Western blotting analysis using Sirtuin 1/SIRT1 (S47) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sirtuin 1/SIRT1 [p Ser47] Antibody (23GB1540)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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