SIN3B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20367
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24691445).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit SIN3B Antibody - BSA Free (NBP2-20367) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-SIN3B Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SIN3B Antibody - BSA Free
![Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367]](https://resources.bio-techne.com/images/products/SIN3B-Antibody-Immunohistochemistry-Paraffin-NBP2-20367-img0005.jpg "Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367]")
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367]
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - MCF7 xenograft, using SIN3B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Immunoprecipitation: SIN3B Antibody [NBP2-20367]](https://resources.bio-techne.com/images/products/SIN3B-Antibody-Immunoprecipitation-NBP2-20367-img0008.jpg "Immunoprecipitation: SIN3B Antibody [NBP2-20367]")
Immunoprecipitation: SIN3B Antibody [NBP2-20367]
Immunoprecipitation: SIN3B Antibody [NBP2-20367] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 ug of SIN3B antibody [N1N2], N-term Western blot analysis was performed using SIN3B antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Western Blot: SIN3B Antibody [NBP2-20367] -
Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SIN3B antibody [N1N2], N-term (NBP2-20367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for SIN3B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SIN3B
Alternate Names
Histone deacetylase complex subunit Sin3b, KIAA0700paired amphipathic helix protein Sin3b, SIN3 homolog B, transcription regulator (yeast), SIN3 homolog B, transcriptional regulator, SIN3 homolog B, transcriptional regulator (yeast), Transcriptional corepressor Sin3b
Gene Symbol
SIN3B
UniProt
Additional SIN3B Products
Product Documents for SIN3B Antibody - BSA Free
Product Specific Notices for SIN3B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.