SET Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33713

Novus Biologicals, part of Bio-Techne
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NBP1-33713
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SET Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SET. The exact sequence is proprietary.

Localization

Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus, nucleoplasm

Specificity

Antibody reactive against recombinant protein, detects immunogen. Further validation in progress.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SET Antibody - BSA Free (NBP1-33713) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-SET Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SET Antibody - BSA Free

Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000
Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract whole cell lysate/extract. B. 30 ug H1299 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug Molt-4 whole cell lysate/extract. F. 30 ug Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translational
Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713]

Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.
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Applications for SET Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SET

SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing : Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

Alternate Names

2PP2A, HLA-DR-associated protein II, I2PP2A, I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPIITAF-I, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, protein SET, SET nuclear oncogene, SET translocation (myeloid leukemia-associated), TAF-IBETA, Template-activating factor I, Template-Activating Factor-I, chromatin remodelling factor

Entrez Gene IDs

6418 (Human)

Gene Symbol

SET

UniProt

Q01105 (Human)

Additional SET Products

Product Documents for SET Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SET Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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