SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25663
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1301 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length SARS-CoV-2 nsp7 recombinant protein
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free (NBP3-25663) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] -
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] - SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm by immunohistochemical analysis. Sample: SARS-CoV-2 (COVID-19) nsp7 transfected 293T cell FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] -
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] - SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.
Western Blot: SARS-CoV-2 nsp7 Antibody (HL1301) [NBP3-25663] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp7
References
1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9
3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39
4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774
Alternate Names
Non-structural protein 7, nsp7 replicase
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp7 Products
Product Documents for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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