Western blot shows recombinant SARS-CoV-2 Nsp7 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 NSP7 Monoclonal Antibody (Catalog # MAB12505) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nsp7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for SARS-CoV-2 NSP7 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant SARS-CoV-2 Nsp7 protein

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSP7

Non-structural protein 7 (NSP7) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV-2 that have had significant impact on humans in addition to strains that have negatively impacted livestock. NSP7 is a small 83 amino acid protein that is highly conserved across coronaviruses (1). The NSP7 monomers are composed of a central core N-terminal tri-helical bundle with an additional short C-terminal helix (2). The monomeric units associate to form a large hexadecameric structure with NSP8 (2) where layers of NSP7 fill the spaces in between NSP8 units. The supercomplexes are stacked to form a channel with electrostatic properties that could allow RNA to pass through the channel to facilitate efficient replication and transcription. The NSP7/NSP8 supercomplex was thereby proposed to function as a primase for the viral RNA-dependent RNA polymerase (RdRp), NSP12 (3). In SARS-CoV-2, the RdRp has been shown to have little activity without NSP8/7 as cofactors (4) making NSP7/NSP8 critical for viral polymerase activity. NSP7 has also been shown to interact with several other viral NSP proteins, including NSP5, NSP9, and NSP13 (5) as well as multiple host cell proteins involved in membrane trafficking, signaling, and electron transport including potential drug targets such as COMT and PTGES (6).

References

Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59.
Zhai, Y. et al. (2005) Nat. Struct. Mol. Bio. 12:980.
Subissi, L. et al. (2014) Antiviral Res. 101:122.
Yin, W. et al. (2020) Science 368:1499.
von Brunn, A. et al. (2007) PLoS One 2:e459.
Gordon, D.E. et al. (2020) Nature 583:459.

Long Name

Non-Structural Protein 7

UniProt

YP_009725303.1

Additional NSP7 Products

Product Documents for SARS-CoV-2 NSP7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for SARS-CoV-2 NSP7 Antibody

For research use only

Citations
Reviews

SARS-CoV-2 NSP7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB12505

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NSP7 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for SARS-CoV-2 NSP7 Antibody

Detection of SARS-CoV-2 Nsp7 by Western Blot.