SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25650

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25650
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1381 expressed in HEK293

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 NSP10 Antibodies »

Product Specifications

Immunogen

Full length SARS-CoV-2 NSP10 recombinant protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free (NBP3-25650) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] - SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] (NBP3-25650) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.
SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Western Blot: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] -

Western Blot: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] (NBP3-25650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 NSP10

SARS-CoV-2 Nonstructural Protein 9 (NSP9) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP9 is 113 amino acids (aa) with a theoretical molecular weight of 12.4 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP9 has 97.3% sequence identity and 99.1% sequence similarity (1). Structurally NSP9 has a N-terminal beta-barrel made of seven beta-strands and a C-terminal alpha-helix (3). In SARS-CoV, NSP9 is important for viral proliferation; it is unclear if NSP9 plays the same role in SARS-CoV-2 but given the high degree of sequence similarity it is quite likely (4). SARS-CoV-2 NSP9 also plays a role in formation of nuclear transport machinery and the extracellular matrix (2). Additionally, NSP9 binds mind bomb 1 (MIB1), an E3 ubiquitin ligase that regulates antiviral innate immune signaling (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Khan, M. T., Irfan, M., Ahsan, H., Ahmed, A., Kaushik, A. C., Khan, A. S., Chinnasamy, S., Ali, A., & Wei, D. Q. (2021). Structures of SARS-CoV-2 RNA-Binding Proteins and Therapeutic Targets. Intervirology. https://doi.org/10.1159/000513686

Alternate Names

GFL, Growth factor-like peptide, Non-structural protein 10

Gene Symbol

ORF1ab

Additional SARS-CoV-2 NSP10 Products

Product Documents for SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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