SARS-CoV-2 NSP10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11049

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MAB11049-100
MAB11049-SP
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1049919

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Product Specifications

Immunogen

E. coli-derived SARS-CoV-2 NSP10 protein
Ala1-Gln139
Accession # YP_009725306.1

Specificity

Detects SARS-CoV-2 NSP10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SARS-CoV-2 NSP10 Antibody

Detection of SARS-CoV-2 NSP10 by Western Blot.

Western blot shows recombinant SARS-CoV-2 NSP10. PVDF membrane was probed with 1 µg/mL of Mouse Anti-SARS-CoV-2 NSP10 Monoclonal Antibody (Catalog # MAB11049) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NSP10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

NSP10 in HEK293 Human Cell line Transfected with NSP10.

NSP10 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with NSP10 (positive staining) and HEK293 human embryonic kidney cell line (wild type, negative staining) using Mouse Anti-SARS-CoV-2 NSP10 Monoclonal Antibody (Catalog # MAB11049) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for SARS-CoV-2 NSP10 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with NSP10

Western Blot

1 µg/mL
Sample: Recombinant SARS-CoV-2 NSP10

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSP10

Non-structural protein 10 (NSP10), is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP10 is a small 139 amino acid protein that forms independent dodecameric structures composed of four identical trimers where each monomer is capable of binding two zinc ions (2, 3) that are coordinated uniquely and has exposed basic residues on the protein's surface consequently allowing RNA-binding (3). The NSP10 sequence is conserved across coronaviruses (4) and is thought to serve as an important multifunctional cofactor in viral replication. NSP10 was shown to interact with other NSP proteins including NSP14 and NSP16 (5). The N-terminus of NSP10 has been shown to interact with NSP14 and proposed to stabilize and regulate the 3'-5' exoribonuclease domain activity while not directly impacting the methyltransferase activity of NSP14 (6,7). A similar region of NSP10 interacts with NSP16; this interaction is required for stabilization of NSP16 and stimulation of its 2'-O-methyltransferase activity (4,7). Consequently, NSP10 is critically involved in the formation of functional viral mRNA capping machinery in coronaviruses and a potential target to reduce viral replication and pathogenesis (8,9). 

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res. 96:59. 
  2. Matthes, N. et al. (2006) FEBS Lett. 580:4143.
  3. Su, D. et al. (2006) J. Virol. 80:7902.
  4. Rosas-Lemus, M. et al. (2020) bioRxiv. In press.
  5. Pan, J. et al. (2008) PLoS One 3:e3299.
  6. Bouvet, M. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:9372.
  7. Bouvet, M. et al. (2014) J. Biol. Chem. 289:25783.
  8. Ma, Y. et al. (2015) Proc. Natl. Acad. Sci. 112:9436.
  9. Wang, Y. et al. (2015) J. Virol. 89:8416.

Long Name

Non-Structural Protein 10

UniProt

YP_009725306.1

Additional NSP10 Products

Product Documents for SARS-CoV-2 NSP10 Antibody

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Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 NSP10 Antibody

For research use only

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