SARS-CoV-2 Envelope Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41061
![ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP2-41061]](https://resources.bio-techne.com/images/products/SARS-CoV-2-Envelope-Antibody-ELISA-NBP2-41061-img0001.jpg "ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP2-41061]")
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Predicted:
SARS-CoV (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody was raised against a peptide corresponding to 10 amino acids near the amino terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 Envelope Antibody - BSA Free
ELISA: SARS-CoV-2 Envelope Antibody - BSA Free [NBP2-41061]
ELISA: SARS-CoV-2 Envelope Antibody [NBP2-41061] - SARS-CoV-2 Envelope Antibody. A direct ELISA was performed using antigen or control peptide as coating antigen and the anti-SARS-CoV-2 Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 32 ng/mL to 2000ng/mL.
Immunohistochemistry: SARS-CoV-2 Envelope Antibody - BSA Free [NBP2-41061] -
Immunohistochemistry: SARS-CoV-2 Envelope Antibody - BSA Free [NBP2-41061] - Validation of SARS-CoV-2 (COVID-19) Envelope in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.Applications for SARS-CoV-2 Envelope Antibody - BSA Free
Application
Recommended Usage
ELISA
1 ug/mL
Immunocytochemistry/ Immunofluorescence
1-3 ug/ml
Immunohistochemistry
1-3 ug/ml
Immunohistochemistry-Paraffin
1-3 ug/ml
Application Notes
This antibody cannot detect envelope recombinant protein in WB.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: SARS-CoV-2 Envelope
References
1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.
2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
3. Uniprot (P0DTC4)
4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144
Alternate Names
2019-nCoV Envelope protein, COVID-19 Envelope protein, E Protein, Envelope, Human Coronavirus Envelope protein, SARSCoV2, SARS-CoV-2 E protein, SARSCoV2 Envelope protein, SARS-CoV-2 Envelope protein, Severe acute respiratory syndrome 2 Envelope Protein
Entrez Gene IDs
QHD43418 (SARS-CoV-2)
Gene Symbol
E
Additional SARS-CoV-2 Envelope Products
Product Documents for SARS-CoV-2 Envelope Antibody - BSA Free
Product Specific Notices for SARS-CoV-2 Envelope Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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