SAMM50 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84509

Novus Biologicals, part of Bio-Techne
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NBP1-84509
NBP1-84509-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAMM50 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLID

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SAMM50 Antibody - BSA Free (NBP1-84509) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-SAMM50 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SAMM50 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509]

Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509]

Immunocytochemistry/Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.
View 8 more images

Applications for SAMM50 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Immunoprecipitation

Reported in scientific literature (PMID:26308446).

Simple Western

1:4

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:4, apparent MW was 56 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAMM50

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAMcomplex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005(PubMed 15644312)).(supplied by OMIM)

Alternate Names

CGI-51, FLJ42905, FLJ99036, OMP85, SAM50, sorting and assembly machinery 50kDa, sorting and assembly machinery component 50 homolog, sorting and assembly machinery component 50 homolog (S. cerevisiae), TOB55, Transformation-related gene 3 protein, transformation-related protein 3, TRG-3FLJ35825, YNL026W

Gene Symbol

SAMM50

Additional SAMM50 Products

Product Documents for SAMM50 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAMM50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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