RSK3 [p Ser360, p Thr356] Antibody (HL3299)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48789
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3299
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr356/Ser360 of human RSK3. The exact sequence is proprietary.
Modification
p Ser360, p Thr356
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RSK3 [p Ser360, p Thr356] Antibody (HL3299) (NBP3-48789) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RSK3 [p Ser360, p Thr356] Antibody (HL3299)
Western Blot: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -
Untreated (-) and treated (+) NIH-3T3 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -
Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -
RSK3 antibody [HL3299] detects RSK3 protein by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RSK3 stained by RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:500.Blue: Fluoroshield with DAPI .Applications for RSK3 [p Ser360, p Thr356] Antibody (HL3299)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RSK3
Long Name
Ribosomal Protein S6 Kinase III
Alternate Names
RPS6KA2
Gene Symbol
RPS6KA2
Additional RSK3 Products
Product Documents for RSK3 [p Ser360, p Thr356] Antibody (HL3299)
Product Specific Notices for RSK3 [p Ser360, p Thr356] Antibody (HL3299)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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