ROCK1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-624
![Western Blot: ROCK1 Antibody [NB100-624]](https://resources.bio-techne.com/images/products/ROCK1-Antibody-NB100-624-img0003.jpg "Western Blot: ROCK1 Antibody [NB100-624]")
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1354) of human Rho-associated, coiled-coil containing protein kinase 1 using the numbering given in Swiss-Prot entry Q13464 (GeneID 6093).
Specificity
NB100-624 is specific for human Rho Kinase Beta protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ROCK1 Antibody - BSA Free
Western Blot: ROCK1 Antibody [NB100-624]
Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).Applications for ROCK1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:5000-1:15000
Application Notes
Use in IHC, IHC-P and ICC/IF reported in scientific literature (PMID 24324133).
Reviewed Applications
Read 2 reviews rated 5 using NB100-624 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ROCK1
Long Name
Rho-associated, Coiled-Coil Containing Protein Kinase 1
Alternate Names
p160ROCK, ROK beta
Gene Symbol
ROCK1
UniProt
Additional ROCK1 Products
Product Documents for ROCK1 Antibody - BSA Free
Product Specific Notices for ROCK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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